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Reconstitution calculator

Work out the concentration, the volume and the number of units to draw up after reconstituting a lyophilised peptide.

An informational tool intended for research. It is not medical advice or a dosage recommendation. Always double-check your calculations. No product is intended for human consumption.
1 · Your inputs
No products found.
mg
ml
The volume of diluent you inject into the vial.
U-100 = 100 units (IU) per ml — the most common.
2 · Result
To draw into the syringe
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Concentration
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mg/ml
Dose per unit (IU)
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Doses per vial
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How does it work?

What is reconstitution?

A lyophilised peptide (powder) is dissolved in bacteriostatic water. The volume of water added determines the final concentration — and therefore the number of units to draw for a given dose.

Concentration

Concentration = amount of peptide (mg) ÷ water added (ml). The more water you add, the more dilute the solution and the more units you draw for the same dose.

Reading the syringe

On a U-100 insulin syringe, “1 ml” corresponds to 100 units (IU). The result tells you how many units to draw, up to the matching mark.

Dilution tip

Choose a volume of water that gives an easy-to-read number of units (neither too small nor beyond the syringe's capacity).

Important warnings — please read

  • For research use only. The products and this calculator are intended for in-vitro research and educational purposes. Nothing here is intended for human or animal consumption.
  • Not medical advice. This calculator is not a substitute for a healthcare professional. No dose is recommended or suggested.
  • Check your calculations. Results are provided “as is”, without any warranty. A single input error (mg/mcg, ml) changes the whole result — always double-check.
  • Handling. Follow the rules on hygiene, sterility and disposal of equipment. Never reuse a needle.
  • Liability. PIK.Supplies accepts no liability for the use of this tool or of the products. You alone are responsible for complying with the laws of your country.